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mediante el sistema CRISPR-Cas9.
All content in this area was uploaded by Fuguo Jiang on May 25, 2017 .
The contributors cover web-based tools and approaches for designing guide RNAs that precisely target genes of interest, methods for preparing and delivering CRISPR-Cas reagents into cells, and ways to screen for cells that harbor the desired genetic changes. © 2016
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Si pensamos en que así como se llevó a cabo con el pez cebra, se puede hacer con cualquier organismo viviente, empezamos a comprender el …
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Download a Free Excerpt from CRISPR-Cas: A Laboratory Manual: Contents and Preface Sample Protocol: Generation of Genetically Modified Mice Using the CRISPR-Cas9 Genome-Editing System Index.
Strategies for optimizing CRISPR-Cas in each system—especially for minimizing off-target effects—are also provided.
Authors also describe other applications of the CRISPR-Cas system, including its use for regulating genome activation and repression, and discuss the development of next-generation CRISPR-Cas tools.
CRISPR-Cas9 is a gene editing technology that uses a combination of (1) an enzyme that cuts DNA (Cas9, a nuclease) and (2) a guiding piece of genetic material (guide RNA) to specify the location in the genome.
Generally, the guide RNA targets and binds to a specific DNA sequence,
Considerando el gran tamaño que los genomas suelen tener y la fa - cilidad con que se hizo, esto es un logro formidable.
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https://doi.org/10.1007/978-1-4939-9170-9, Springer Science+Business Media, LLC, part of Springer Nature 2019, Rapid Quantitative Evaluation of CRISPR Genome Editing by TIDE and TIDER, Fast and Quantitative Identification of Ex Vivo Precise Genome Targeting-Induced Indel Events by IDAA, Functional Evaluation of CRISPR Activity by the Dual-Fluorescent Surrogate System: C-Check, CRISPR-Cas9 Delivery by Artificial Virus (RRPHC), Production and Validation of Lentiviral Vectors for CRISPR/Cas9 Delivery, Rapid and Simple Screening of CRISPR Guide RNAs (gRNAs) in Cultured Cells Using Adeno-Associated Viral (AAV) Vectors, Electroporation-Based CRISPR/Cas9 Gene Editing Using Cas9 Protein and Chemically Modified sgRNAs, Efficient Gene Editing of Human Induced Pluripotent Stem Cells Using CRISPR/Cas9, Editing the Genome of Human Induced Pluripotent Stem Cells Using CRISPR/Cas9 Ribonucleoprotein Complexes, Conditional Gene Knockout in Human Cells with Inducible CRISPR/Cas9, CRISPR/Cas9 as a Genome Editing Tool for Targeted Gene Integration in CHO Cells, Rapid and Efficient Gene Deletion by CRISPR/Cas9, CRISPR/Cas9-Mediated Gene Tagging: A Step-by-Step Protocol, Gene Editing in Primary Cells of Cattle and Pig, CRISPR Gene Therapy of the Eye: Targeted Knockout of, In Vivo Editing of the Adult Mouse Liver Using CRISPR/Cas9 and Hydrodynamic Tail Vein Injection, CRISPR-Based Lentiviral Knockout Libraries for Functional Genomic Screening and Identification of Phenotype-Related Genes.
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Written for the highly successful. Cold Spring Harbor Laboratory Press Bookstore, Sample Protocol: Generation of Genetically Modified Mice Using the CRISPR-Cas9 Genome-Editing System, Introduction to Protein-DNA Interactions: Structure, Thermodynamics, and Bioinformatics, Quickstart Molecular Biology: An Introduction for Mathematicians, Physicists, and Computational Scientists, RNA Worlds: From Life's Origins to Diversity in Gene Regulation. Free shipping offer applies to direct website purchases by individual U.S. and Canada customers only.
The book is thus an essential laboratory resource for all cell, molecular, and developmental biologists, as well as biochemists, geneticists, and all who seek to expand their biotechnology toolkits. Xi Xiang, Conghui Li, Xi Chen, Hongwei Dou, Yong Li, Xiuqing Zhang et al. It includes step-by-step protocols for applying CRISPR-Cas-based techniques in various systems, including yeast, zebrafish, Drosophila, mice, and cultured cells (e.g., human pluripotent stem cells).
Chapter 3 is available open access under a CC BY 4.0 license via link.springer.com.
Petra Vochozkova, Kilian Simmet, Eva-Maria Jemiller, Annegret Wünsch, Nikolai Klymiuk, Kristian Alsbjerg Skipper, Jacob Giehm Mikkelsen, Andreas Holmgaard, Sidsel Alsing, Anne Louise Askou, Thomas J. Corydon, Francesco Niola, Frederik Dagnæs-Hansen, Morten Frödin, Emil Aagaard Thomsen, Jacob Giehm Mikkelsen, This detailed volume guides readers through strategic planning and user-friendly guidelines in order to select the most suitable CRISPR-Cas system and target sites with high activity and specificity. TO ORDER Toll Free 888.530.0801 Direct 608.441.2852 www.mirusbio.com The CRISPR/Cas9 RNP Complex. book series
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Methods in Molecular Biology
Methods covering CRISPR gRNA design, CRISPR delivery, CRISPR activity quantification (indel quantification), and examples of applying CRISPR gene editing in human pluripotent stem cells, primary cells, gene therapy, and genetic screening are included.
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